Misfolding & Disease

Amyloidogenesis and Prion Propagation

Summary

Amyloidogenesis is the process by which proteins assemble into ordered, insoluble fibrils with a cross-β structure. Prion propagation is a specific subset where the misfolded state becomes infectious and self-propagating.

Key Points

  • 1Amyloid fibrils have cross-β structure with steric zippers
  • 2Prions are infectious proteins that template their conformation
  • 3Prion strains arise from distinct conformations of the same sequence
  • 4Prion-like mechanisms drive progression of major neurodegenerative diseases

Amyloidogenesis and prion propagation represent extremes of protein misfolding where alternative conformations become self-perpetuating.

Amyloid Structure

Amyloid fibrils share a common cross-β architecture:

The Cross-β Spine

- β-strands run perpendicular to the fibril axis

  • Creates continuous β-sheets along the fibril length
  • Backbone hydrogen bonds stabilize the structure
  • Steric Zippers

    At the atomic level, amyloid is stabilized by steric zippers:

  • Dry, tightly interdigitated interfaces between β-sheets
  • Side chains pack like interlocking teeth
  • Excludes water from the interface
  • Creates extraordinary stability
  • Prion Propagation

    Prion diseases represent a unique form of pathology where misfolding is infectious.

    The Protein-Only Hypothesis

    Stanley Prusiner's revolutionary concept:

    - The pathogenic agent is a protein (PrP^Sc), not a virus or bacterium

    - PrP^Sc acts as a conformational template

  • Native PrP^C is recruited and converted to the misfolded form
  • No nucleic acid is required for transmission
  • The Prion Cycle

  • PrP^Sc binds to native PrP^C
  • PrP^C is converted to the PrP^Sc conformation
  • Fibrils grow by incorporation
  • 4. Fragmentation creates new seeds

  • Seeds spread to new cells/organisms
  • Prion Strains

    A remarkable phenomenon demonstrating conformational diversity:

    - A single amino acid sequence can adopt multiple distinct prion conformations

  • Each "strain" produces different disease phenotypes
  • Strains are faithfully replicated during propagation
  • Explains clinical heterogeneity in prion diseases
  • Species Barriers

    Prion transmission between species is limited by:

  • Sequence compatibility between seed and substrate
  • Structural fit of the templating surface
  • Can be overcome by adaptation over serial passages
  • Prion-Like Mechanisms in Common Diseases

    The prion paradigm now extends beyond classical prion diseases:

    - Alzheimer's: Aβ and tau spread in prion-like fashion

    - Parkinson's: α-synuclein propagates between neurons

    - ALS: SOD1 and TDP-43 show prion-like properties

    This "prion-like" spreading explains the stereotyped progression patterns of neurodegenerative diseases.